Equipment

Equipment Staff in charge Description and resources
Laser Microdissection
Leica AS-LMD
                                              Christophe KLEINDissection of specific subpopulations of tissue cells under direct microscopic visualization

Cryostat
Leica CM 305S

Kevin GARBIN

Frozen block sectioning. Sections from 5µm thickness

 Microtome
Leica RM2145
 Kevin GARBINParaffin block sectioning. Sections from 3µm thickness 

Paraffin embedding
Leica ASP300 and HistoCore Arcadia

Kevin GARBIN

Automated Dehydration and impregnation of fixed and bon-fixed tissue samples.

Autostainer
Roche Ventana Discovery XT

Kevin GARBIN
Capacity of 30 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application.
 Autostainer DAKO 48L
Kevin GARBIN 
Capacity of 48 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application. unmasking and dewaxing treatments must be done externally prior to use the equipment. A TintoRetriever Digital Pressure Cooker with Thermometer is available to perform unmasking and dewaxing
 Autostainer
Leica Bond RX
 Kevin GARBINAutomated autostainer suitable for IHC, ISH, FISH, multiplexing experiments. Slides can be treated independently
 Equipment Staff in charge Description and resources

Confocal Microscope 
Zeiss LSM 710
                              
Christophe KLEIN
6 excitation wave length: 405, 458, 488, 514, 561 et 633nm.
Spectral unmixing: detects up to 10 parameters simultaneously.
Application

Fluorescence microscope
Zeiss Axiovert 200M

Christophe KLEIN
Equiped with 4 detection filters: DAPI (Ex=365nm; Em=445nm), FITC (Ex=470nm; Em=525nm), TRITC (Ex=545nm; Em=605nm), Cy5 (Ex=640nm; Em=690nm)
Application
 Slide Scanner Zeiss
Axio Scan Z1
 Christophe KLEINDigitalization x10, x20,  x40Automated brightfield and multichannel fluorescence (up to 5 biomarkersk, or 4 biomarkers nuclei staining)coupled with Zen software Image analysis can be performed with Visiopharm software (-cell counting, measure of areas of cells…). Analysis workstations available.
Laser Microdissection Leica AS-LMDChristophe KLEINDissection specific subpopulations of tissue cells Under direct microscopic visualization
Spectral microscope Perkin Elmer
Mantra

Christophe KLEIN
Can acquire um to 7 fluorochromes or 4 immunohistochemical stains.
Distinctly differentiate autofluoresence.
Perkin Elmer InForm worstation allows automatic cell recognition and couting function of their phenotype.
Application
 ELISPOT
CTL Immunospot

Estelle DEVEVRE 
 Enzymatic and fluorescence detection up to 2 colors
Application
 EquipmentStaff in chargeDescritopn and resources
Multi-analyte assay
Luminex
FlexMap3D 
Hélène FOHRER-TING
Estelle DEVEVRE
Rapid measurment of maximum 500 analytes per sample simultaneously with  96 and 384 well plates.
Application
 
Bench top cytometer BD LSR II

Hélène FOHRER-TING
Estelle DEVEVRE  
10 parameters on 3 lasers. Compatible with 12x75mm polystyrene FACS tubes. ref 352054.Complete Description
Optics
Application
 Bench-top cytometer
BD Fortessa X20
Hélène FOHRER-TINGEstelle DEVEVRE  18 parameters on 4 lasers.
Compatible with 12x75mm polystyrene FACS tubes (ref 352054).
Complete description
Optics
Application 
 FACS cell sorter
BD
FACS Aria III 
Hélène FOHRER-TING
Estelle DEVEVRE 
16 parameters on 4 lasers. Sort up to 4 populations simultaneouslyComplete description
Optics
Application 

FACS cell sorter
BD Influx

Hélène FOHRER-TING
Estelle DEVEVRE
17 parameters on 4 lasers. Can sort up to 4 populations simultaneously. Compatible with 12x75mm polypropylene FACS tubes (Ref. 352063)Complete description
Optics
Application 

 Automated magnetic cell sorter Miltenyi Biotec Automacs Pro

Hélène FOHRER-TING
 
Fully automated cell labeling, and magnetic cell sorting. Can program up to 6 independent sorting. Doubled column capacity compared to manual selection. 
 Application   
 TECHNOLOGYOPERATORDESCRIPTION AND RESSOURCES 

Multi-analyte assay Luminex
Hélène FOHRER-TING
Estelle DEVEVRE
More sensitive than ELISA, Luminex technology is the right choice. When no satisfying commercial solution is available, we can help you set-up an assay. 

Flow Cytometry 
 
Hélène FOHRER-TING
Estelle DEVEVRE
We can provide you with a complete flow cytometry data analysis service . From raw data to final figures for publication
Softwares available: DIVA, Flow Jo, Spice, R
Application 
Cell ImagingChristophe KLEINWe can provide you with a complete image analysis service. From raw data to final figures for publication
Softwares available: Image J, Visopharm, Zen