Inflammation and Cancer

The tumor microenvironment of solid tumors is highly complex, heterogenous and dynamic, and strongly influences clinical outcome and response to treatments. The goals of the Inflammation and Cancer groupare to characterize the host cellular and molecular events that shape the tumor microenvironment and how this influences response to anti-tumor treatments, and to determine how viral infections reprogram the tumor microenvironment. We focus our studies on lung and renal cancer, and on lymphoma, in which we analyze immune cell infiltration and organization (phenotypes, densities, co-localizations and tertiary lymphoid structures), fibroblasts and extracellular matrix and tumor vasculature. We also study immune checkpoint inhibitors (ICI)-related irAEs, to identify B cell subsets and Abs involved in these effects. All these questions are addressed in large cohorts of patients and 3D heterotypic spheroid and tumoroid models, using transcriptomic approaches (RNAseq, scRNAseq, spatial transcriptomics), highly multiplex IF, 3D confocal live imaging, and bioinformatic approaches.

Our current main questions are:

– To decipher the cellular and molecular mechanisms leading to immune cell exclusion in the TME (Isabelle Cremer)

-To characterize the TME by spatial in situ analysis of immune clusters, metabolic pathways in tumor and immune cells and molecular alterations (Diane Damotte, Audrey Lupo, Marco Alifano).

– To deeply characterize how viruses reprogram the tumor microenvironment leading to resistance to chemotherapy, increased tumor invasiveness and angiogenesis (Isabelle Cremer)

– To determine the antitumor effects of cold atmospheric plasma in mice models of lung cancer (Isabelle Cremer)

– To identify mechanisms leading to autophagy that involves extracellular microvesicles and its impact in anti-tumor immunity (Pierre-Emmanuel Joubert).

– To characterize the molecular signature of aberrant vasculature in renal cell carcinoma and to study the functionality of these aberrant structures (Catherine Monnot).

– To study  immune checkpoint inhibitors (ICI)-related irAEs and characterize the functional links between clinical response to ICIs and appearance of auto-immune manifestations (Sophie Sibéril).

Permanent Members

  • Isabelle Cremer (PU-EX Sorbonne Université)
  • Catherine Monnot (CR-HC INSERM)
  • Sophie Sibéril (MCU-HC Sorbonne Université)
  • Pierre-Emmanuel Joubert (MCU Sorbonne Université)
  • Diane Damotte (PU-PH Université Paris Cité)
  • Marie Wislez (PU-PH Université Paris Cité)
  • Audrey Lupo (MCU-PH Université Paris Cité).

Pubmed key

Cremer I OR Damotte D OR Alifano M OR Sibéril S OR Joubert PE OR Wislez M

Keywords

Tumor microenvironment, immune cells, tertiary lymphoid structures, lung cancer, renal cancer, lymphoma, viral infections, tumor vasculature, autophagy, extracellular vesicles, auto-Abs, immunotherapy, chemotherapy, cold atmospheric plasma.

Researchers info

Isabelle CREMER :
isabelle.cremer@sorbonne-universite.fr
Orcid: 0000-0002-0963-1031
Twitter : @CremerIsabelle

Diane DAMOTTE :
diane.damotte@aphp.fr
Orcid: 0000-0001-5355-3045
Twitter : @DianeDamotte

Marie WISLEZ :
marie.wislez@aphp.fr
Orcid: 0000-0001-7518-7859
Twitter : @MarieWislez

Marco ALIFANO :
marco.alifano@aphp.fr
Orcid: 0000-0001-7518-7859
Twitter : @AlifanoMarco

Karen LEROY :
karen.leroy@aphp.fr
Orcid: 0000-0002-4379-0140

Audrey LUPO :
audrey.lupo@aphp.fr
Orcid: 0000-0002-7196-2114

Sophie SIBERIL :
sophie.siberil@bdiagne
Orcid : 0000-0003-0184-2886

Pierre-Emmanuel JOUBERT :
pierre-emmanuel.joubert@agathe-yacoumas
Orcid : 0000-0001-9859-6479

Catherine MONNOT :
catherine.monnot@inserm.fr
Orcid : 0000-0003-1334-7348